Analysis Note

ControlA431 cell lysate

Application

Detect erbB4 using this Anti-erbB4 Antibody, clone HFR1/2G4 validated for use in WB, IP, IH(P).

Research CategorySignalingApoptosis & Cancer

Research Sub CategoryGrowth Factors & Receptors

Immunoprecipitation: A previous lot of this antibody was used in IP.

Immunohistochemistry: Effective for formalin-fixed, paraffin-embedded sections.
Optimal working dilutions must be determined by end user.

Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of erbB-4/HER-4 Monoclonal Antibody: DCIS and Pancreatic Cancer

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

v-ErbB was identified as the viral counterpart of the EGF Receptor, and its relatives have adopted this nomenclature (or the HER nomenclature for human proteins). These proteins are activated by Heregulin, Neuregulin and Amphiregulin. ErbB-2/HER-2, also known as neu, is amplified in large numbers of highly aggressive breast cancers, but all the family members are capable of heterodimerization, and together activate downstream signaling pathways. ErbB-3 has a natural "substitution" at the codon which is an invariant Lys residue at the ATP-binding site of all protein kinases. It is therefore inactive as a kinase, and can only become phosphorylated by heterodimerization with another erbB-family member. All members of this family, when Tyr-phosphorylated, can nucleate the formation of signaling complexes through adapter proteins.

Immunogen

Epitope: Cytoplasmic domain of the protein, residues 1250-1264.

Synthetic peptide sequence RSTLQHPDYLQEYST from the cytoplasmic domain of the protein, residues 1250-1264.

Linkage

Replaces: 04-348

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified

Format: Purified

Purified mouse monoclonal IgM in 0.02 M PB with 0.25 M NaCl, pH 7.6, 0.01% Sodium Azide.

Quality

Routinely evaluated by Western Blot on A431 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected C-ERB-4 on 10 µg of A431 lysates.

Specificity

Reactive with human c-erB-4/Her 4 cytoplasmic domain of the protein (residues 1250-1264), a member of the EGF receptor (type 1) family involved in cell growth regulation. Expression or function may be altered in human cancers.

Storage and Stability

Stable refrigerated at 2-8°C in undiluted aliquots for up to 1 year from date of receipt.

Target description

~147 kDa